An antibody is a protein that is used by the immune system to identify and neutralize foreign objects, such as bacteria, viruses, and other foreign cells. They recognize specific antigens that bind to antibodies with high affinity.
In terms of its mechanism of action, the antigen usually binds to the variable region of the Y-shaped structure of the antibody, which consists of two heavy chains and two light chains. Each of these chains is held together by a stable disulfide bridge. Boster Bio featured products can provide the best antibody services.
Antibodies fall under the IgG subtype, which makes up about 85% of the antibodies found in blood serum in the bloodstream. The other isotypes are much less common and occur only in certain tissue types. For this reason, the IgG antibody is the most important isotype used in therapy.
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Antibodies interact with antigens by reversible non-covalent bonds and serve to determine the specificity and sensitivity of their interactions.
In the mouse (or the rabbit hybridoma method developed later in the 1990s), the animal is first injected with the antigen. The animal's immune system will start producing antibodies in response to this antigen. Several rounds of screening are then performed to check the antigen specificity of the antibody.
Once the titer was deemed sufficient, B cell screens were removed from the animals and splenic B cells were infused with myeloma cells to create immortal cell lines.
Rabbit monoclonal antibodies (mAbs) have several advantages over mice. The immune system of rabbits is more complex than that of mice and therefore tends to produce antibodies with higher specificity and affinity. In addition, rabbit mAbs are often recommended when experiments do not produce an adequate immune response from the mouse system.